Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoshinaga, T.; Sano, S.
    Coproporphyrinogen oxidase. I. Purification, properties, and activation by phospholipids (1980), J. Biol. Chem., 255, 4722-4726.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.3 2-mercaptoethanol slight activation Bos taurus
1.3.3.3 cardiolipin 2.2fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 DTT slight activation Bos taurus
1.3.3.3 L-alpha-lysophosphatidylcholine 4.4fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 L-alpha-lysophosphatidylethanolamine 3.4fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 L-alpha-phosphatidyl-DL-glycerol 2.7fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 L-alpha-phosphatidyl-L-serine 2.8fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 L-alpha-phosphatidylcholine 3.8fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 L-alpha-Phosphatidylethanolamine 3.9fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 Lipids bovine liver lipids, 3.6fold activation at 1 mg/ml Bos taurus
1.3.3.3 phosphatidate 2.3fold activation at 0.5 mg/ml Bos taurus
1.3.3.3 Triton X-100 concentration-dependent activation up to 2.8fold Bos taurus
1.3.3.3 Tween 20 2.4 fold activation at 0.2%, v/v Bos taurus
1.3.3.3 Tween 40 2.2fold activation at 0.2%, v/v Bos taurus
1.3.3.3 Tween 60 2.3fold activation at 0.2%, v/v Bos taurus
1.3.3.3 Tween 80 2fold activation at 0.2%, v/v Bos taurus
1.3.3.3 Tween 85 1.8 fold activation at 0.2%, v/v Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.3 cetyltrimethylammonium chloride 86% inhibition at 0.05% Bos taurus
1.3.3.3 SDS 84% inhibition at 0.05%, w/v Bos taurus
1.3.3.3 sodium deoxycholate 71% inhibition at 0.05%, w/v Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.3 0.018 0.025 Coproporphyrinogen-III presence of phospholipids Bos taurus
1.3.3.3 0.025
-
 Coproporphyrinogen-III crude extract Bos taurus
1.3.3.3 0.048
-
 Coproporphyrinogen-III purified enzyme Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.3 additional information no metal content Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.3 71600
-
 1 * 71600, SDS-PAGE Bos taurus
1.3.3.3 74000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ Bos taurus highly specific for oxygen as electron acceptor protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 to homogeneity, chromatography steps Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.3 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.3 0.12
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.3.3.3 4°C, 0.1 M Tris-HCl, pH 7.4, 50% saturation ammonium sulfate, 2 weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ highly specific for oxygen as electron acceptor Bos taurus protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.3 monomer 1 * 71600, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.3 7.4
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.3 6.4 8.4 80% of the maximum activity within Bos taurus